Modified Giolitti and Cantoni Broth (ISO) is used for the detection and enumeration of coagulase-positive staphylococci, including Staphylococcus aureus, from food and animal feeding stuffs using the Most Probable Number technique according to ISO 6888-3:2003. This versatile medium can be used with or without an agar/paraffin plug on the surface, depending upon the testing regime employed.
Modified Giolitti and Cantoni Broth is optimised for samples where staphylococci may be stressed and/or present only in low numbers. Their growth is promoted by the inclusion of sodium pyruvate, glycine and high concentration mannitol. Potassium tellurite, which inhibits other Gram-positive organisms, and lithium chloride, inhibitory to Gram-negative bacilli, provide the necessary selectivity.Tellurite also serves as an indicator for coagulase-positive staphylococci, its reduction by these organisms resulting in a blackening of the broth or the presence of a black precipitate.
The introduction of this Modified Giolitti and Cantoni Broth (ISO) enhances Lab M’s range of media for the isolation of coagulase-positive staphylococci in accordance with ISO 6888-3:2003.
Lab M
