Easy-to-use empty columns for process development and preparative chromatography

GE Healthcare HiScale pressure-stable columns are optimized for process development and preparative chromatography. The empty columns are available in inner diameters of 16, 26 and 50 mm and in lengths of 20 and 40 cm. The columns offer an easy-to-use design, providing reproducibility, robustness and process control.

HiScale columns are pressure stabile up to 20 bar, ensuring compatibility with modern BioProcess media such as MabSelect and Capto.

The ability to axially compress the gel bed and a non-rotating plunger mechanism enable a wide range of packing protocols.The measurement scale on the column and user-friendly, ergonomic design enable precise packing, thereby increasing process control and reproducibility. The QuickLock mechanism of the end caps facilitates column handling and cleaning. HiScale can be used with nearly all organic solvents commonly used in liquid chromatography of macromolecules.

HiScale columns were developed for scale-up during process development, and enable packing with high-flow agarose media which require higher pressure. The columns are suitable for use with the new ÄKTA avant 25 and ÄKTA avant 150 systems for process development and scale-up. Both systems exploit the advantages of modern BioProcess chromatography media, enabling smooth development of scalable chromatographic processes for biopharmaceutical production.