HiScreen columns provide a solution for fast, easy separations in a convenient format. The columns are packed with robust media, allowing repeated use with reproducible results. The 10 cm bed height is suitable for method optimization and parameter screening (e.g. for selectivity and capacity), and the columns are easily connected in series to give a 20 cm bed height.
HiScreen columns have a small bed volume (4.7 ml), reducing the cost of sample and buffer consumption. In addition, the columns are made of biocompatible polypropylene that does not interact with biomolecules.
The four new columns are available prepacked with the following chromatography media:
* Ni Sepharose 6 Fast Flow (Ni FF)
Purifying histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC) continues to grow in popularity. Nickel (Ni2+) is the most used metal ion in such IMAC purifications.
* Blue Sepharose 6 Fast Flow (Blue FF)
Cibacron Blue 3G is covalently attached to Sepharose 6 Fast Flow matrix. The blue dye binds many proteins, such as albumin, interferon, lipoproteins, and blood coagulation factors, also several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors, such as NAD+.
* DEAE Sepharose Fast Flow (DEAE FF)
Ion exchange chromatography medium and weak anion exchanger.
* Butyl Sepharose High Performance (Butyl HP)
Hydrophobic interaction chromatography medium, with small bead size resulting in high resolution and sharp peaks.
HiScreen columns are compatible with peristaltic pumps and chromatography systems such as ÄKTA™ design, and are part of GE Healthcare’s process development platform, which offers tools to support all phases from condition screening (i.e. 96-well PreDictor plates) to full-scale manufacturing. The same BioProcess media is used in all formats, enabling easy and reproducible scale-up.
