Mass spectrometry (MS), especially selected reaction monitoring/multiple reaction monitoring (SRM/MRM), has become routine for proteomics, metabolomics, and biomarker validation. SRM and MRM assays conducted on triple quadrupole instruments can be coupled to liquid chromatography (LC) for analysis of complex proteome digests.
For successful execution of SRM/MRM experiments several factors require consideration. These include ready access to appropriate parameters such as peptide choice and transition choice, reproducible and specific sample preparation setup, and optimal use of the mass spectrometer to achieve the highest sensitivity possible.
SRM Atlas, an informatics resource, facilitates setup of SRM/MRM quantification assays of targeted proteins by providing access to these parameters. Created by ordering more than 150,000 synthetic peptides based on 20,300 proteins, the resource generated high-resolution accurate mass spectra on Q-TOF LC/MS systems to create MS/MS profiles for peptide library searching and fragmentation data for the SRM Atlas.
On Thursday, December 16, 2010 (Time: 1:00 pm EST, 10:00 PST), Agilent Technologies Inc is sponsoring a webinar, The SRM Atlas and Multiplexed MRM Protein Assays.
During this webinar you will learn about combining automated robotic sample preparation, SISCAPA sample preparation, and MRM assay setup with transitions from the SRM atlas as well as increasing sensitivity gains from iFunnel-enabled triple quad LC/MS systems to develop practical assays for differential proteomic analysis, biomarker validation, or biological pathway analysis.
Panelists include: Robert Moritz, PhD, Professor and Director of Proteomics, Institute for Systems Biology; Leigh Anderson, PhD, Founder and Chief Executive Officer, Plasma Proteome Institute; Christine Miller, Senior Application Scientist, Agilent Technologies.
A live Q session will follow the presentations, offering you a chance to pose questions to our expert panelists.
Registration is free at www.genengnews.com/proteinquant
