Providing user guidance on the entire qPCR process - from RNA isolation to data analysis - the manual covers the basics of experimental set-up, performance and analysis. Specific information on 5’ nuclease assays, including re-suspensions and qPCR protocols are also supplied, as well as a troubleshooting section which discusses commonly encountered issues.
The entire document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR1.
IDT offers a broad range of PrimeTime qPCR assay products including customized primers and probes, in addition to the free online design suite - SciTools.
1. Bustin, S.A. et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem, 2009. 55(4): p. 611-22.
