Tuesday 25 January 2011

AnaSpec offers α & β-Synuclein recombinant proteins

AnaSpec, Eurogentec Group, is offering two recombinant proteins for use in Neurodegenerative Disease Research, α-synuclein (SNCA) and β-synuclein (SNCB). Both α-synuclein and β-synuclein are expressed and purified from E. coli. α-Synuclein is a 140-amino acid long recombinant protein (GenBank Accession # NP_000336) and β-synuclein is 134-amino acid long (GenBank Accession # NP_003076).

α-Synuclein (SNCA) and β-synuclein, along with γ-synuclein (SNCG), are members of the synuclein family of small proteins expressed primarily in neural tissue and in some tumours1. Synuclein proteins, found only in vertebrates, possess a highly conserved N-terminal domain, with a variable number of 11-residue repeats and a less conserved C-terminal, with a preponderance of acidic residues1. α-Synuclein is used in Parkinson’s disease (PD) research2 and β-synuclein has been shown to induce mild experimental autoimmune encephalomyelitis (EAE) in Lewis rats3. These proteins can be used in applications such as ELISA, western blots and microscopy imaging.

References

1. George, JM. Genome Biol. 3, reviews 3002.1 (2002).
2. MG. et al. Nature 388, 839 (1997).
3. Sung, Y-H. et al. Protein Sci 15, 1162 (2006).
4. Trojanowski, JQ. & VM. Lee, Ann. N. Y. Acad. Sci. 991, 107 (2003).
5. Masliah, E., et al. Science 287, 1265 (2000).
6. Van Der, PH, et al. J. Neurosci. 20, 6021 (2000).
7. Feany, MB. & WW. Bender, Nature 404, 394(2000).
8. Weinreb, PH., et al. Biochemistry 35, 13709 (1996).
9. Rivers, RC. et al. Protein Sci 17, 887 (2008).
10.George, JM. Genome Biol 3, 3002.1 (2001).
11. ruening, W. et al. Am Cancer Soc 88, 2154 (2000).

AnaSpec, Eurogentec Group