Wednesday, 19 January 2011
Method uses GC-MS/MS to increase number of pesticides detectable in single injection
Other commonly used techniques require multiple injections in order to comply with increasingly stringent regulations. The new method, which uses the Thermo Scientific Quantum GC, can acquire 3,000 timed selective reaction monitoring (SRM) transitions per run, increasing sample throughput while ensuring accuracy in a matrix. The method, detailed in an application note entitled "Rapid Analysis of 303 Pesticide Residues in Green Bean Using Triple Quadrupole GC-MS/MS," is available via www.thermoscientific.com/303.
Pesticides used to prevent, destroy and control pests on food crops often leave residues on agricultural products that pose risks to consumers. Effects range from short-term health problems such as headaches and dizziness to long-term damage to the nervous system and, in some cases, cancer.
Gas chromatography (GC) with element-selective detectors and single quadrupole gas chromatography/mass spectrometry (GC/MS) are techniques commonly used for GC-amenable pesticide residue analysis in laboratories. As the use of pesticides increases and maximum residue levels (MRLs) set by regulations become more stringent, labs are requiring alternatives to existing techniques that require one sample be analyzed using several injections and multiple methods. The new Thermo Fisher Scientific GC-MS/MS method enables the rapid screening and confirmation of more than 1000 pesticides in just one injection, increasing productivity and, by delivering results faster, safeguarding human health. The method is designed to facilitate compliance with the European Council Directive 96/23/EC, which requires at least two SRM transitions per compound.
In this method, scientists analyzed 303 pesticides in green beans in a single run using the TSQ Quantum GC under timed-SRM mode. The timed-SRM function enabled the acquisition of a total of 652 transitions. The dwell time of each transition was automatically maximized for each compound to give the best sensitivity for all pesticides. Using this method, linearity and recoveries are achieved within industry requirements and sensitivity can easily reach 0.0040 mg/kg for all pesticides in green beans. The new method demonstrates that timed-SRM mode is an extremely effective method for screening and determining large amounts of pesticides at low levels in the sample matrix.
Thermo Fisher Scientific