Tuesday, 25 January 2011

Optimized insect cell transfection reagent

Oxford Expression Technologies, th specialist provider of baculovirus-based protein expression solutions, has launched baculoFECTIN. This new non-toxic transfection reagent has been optimized for use in insect cells and proven to produce greater levels of transfection – leading to high level protein expression.

The transfection spectrum comprises various established cell lines and primary cells. baculoFECTIN consists of two components, a positively charged polymer with DNA-binding capacity that is embedded into a novel porous nanoparticle. The complete nanoparticle complex protects the bound DNA from degradation by nucleases. Only the DNA nanoparticle complex with its unique size can be preferentially taken up by the cells. Within the cells, the released DNA-nanoparticle complexes are protected against DNA degradation.

“The main benefits of baculoFECTIN compared with lipid-based products are that it has a quick, effective and easy protocol, from co-transfection to high titre seed stock; it is non-toxic so cell health and viability is maintained; and the nanoparticle/polymer duplex optimizes entry into cells and stabilizes the DNA within transfected insect cells,” said Richard Broadhead, sales and marketing manager at Oxford Expression Tehnologies. “Stable for up to a year, baculoFECTIN is the perfect companion to our leading baculovirus expression system flashBAC. It is ideal for high-throughput transfection experiments.”

Oxford Expression Technologies