Monday, 28 February 2011

First Zebrafish specific antibodies

AnaSpec, Eurogentec Group is pleased to be the first in the industry to establish a new product line of zebrafish specific (Z-Fish) antibodies to support the increasing needs of researchers.

In collaboration with the University of Utah, Salt Lake City, Utah, AnaSpec's Z-Fish polyclonal anti-lysozyme was found to completely overlap with mpo: GFP labeled neutrophils. This indicates that lysozyme is a neutrophil marker in zebrafish. Lysozyme C (lyz), a cationic glycoside hydrolase, breaks down N-acetyl muramic b-1, 4 N-acetyl glucosamine linkages in the bacterial cell wall1-2. In zebrafish, this enzyme is expressed exclusively in myeloid cells2 and is used for hematopoietic developmental studies in zebrafish. This antibody was raised using a synthetic peptide derived from the intermediate region of zebrafish lysozyme protein (GenBank accession # NP_631919.1).

AnaSpec's expanding list of Z-Fish antibodies includes targets used in transcriptional regulation, signal transduction, apoptosis and autophagy, cell cycle control, cytoskeleton, glucose and energy metabolism, and immunity research. Antibodies were verified in ELISA. Many were verified in western blots using zebrafish lysates. As well, these Z-Fish antibodies are predicted to work in IHC, many of which have good IHC results. Antibodies are supplied in 150 ul size, this amount is enough for 15 mini gels.

1. Gordon, S. et al. J Exp Med 139(5), 1228 (1974).
2. Kitaguchi, T. et al. Mech Dev 126, 314 (2009).

AnaSpec, Eurogentec Group