Thursday, 24 February 2011

Simplifying the synthesis of complex genes

Integrated DNA Technologies (IDT), the leader in oligonucleotide synthesis, offers a completely confidential custom gene synthesis service. By manufacturing any genes, including hard-to-clone sequences, IDT provides a cost-effective service, eliminating the outlay for construction, cloning, and sequencing. In addition, each gene undergoes design, review and consultation, codon optimization, and sequence verification to provide guaranteed yields of 2µg of purified DNA. As standard, all genes are constructed using Ultramers, the highest fidelity next generation synthesis technology, and are all shipped in a plasmid cloning vector, ready for use.

With the ability to clone from 50 bases up to 5kb, IDT can sequence almost any gene. Unless specified otherwise, all genes are delivered in the proprietary pIDTSmart vector, which has been engineered to remove common restriction sites and does not contain a promoter within the cloning region. With the choice of a kanamycin or ampicillin cassette, users can select their preferred resistance. IDT also has the ability to sub-clone into almost any custom supplied vector.

Integrated DNA Technologies (IDT)