Monday, 14 March 2011

PrimeTime Pre-design qPCR assays increase accuracy and efficiency

Integrated DNA Technologies (IDT), a leader in oligonucleotide synthesis, will be exhibiting at the qPCR 2011 show in Munich, Germany from 28th March to 1st April. Dr Scott Rose, Director of Molecular Genetics at IDT will deliver a talk, entitled ‘PrimeTime Pre-designed qPCR Assays using ZEN Internal Dark Quencher Chemistry’.

Well-designed qPCR assays require the careful consideration of primer placement, specificity, avoidance of SNPs, oligo interactions and accurate Tm calculations. In order to meet these requirements, IDT now offers PrimeTime Pre-designed qPCR Assays for all genes in the human, mouse and rat genome. These assays avoid any cross-reactivity within that genome, known SNPs and primer interactions, while providing full disclosure of all sequence information, as recommended by MIQE guidelines1. By including the ZEN double-quenched probes, these assays provide outstanding levels of sensitivity, with the added security of knowing precise probe location.

IDT’s ZEN double-quenched probe technology increases the accuracy and reliability of 5’ nuclease qPCR experiments by positioning an internal ZEN quencher 9 bases from the 5’ fluorophore. When combined with the standard 3’ quencher, this significantly decreases background fluorescence and increases sensitivity. The initial background fluorescence signal is therefore much lower, making subsequent changes easily detectable. As a result low background is obtained, even at 40 base pairs or longer.

1.The MIQE Guidelines – Minimum information for publication of quantitative real time PCR experiments. Available at:

Integrated DNA Technologies (IDT)